The key sphingolipids-monoglycosylceramide (cerebroside) and phosphosphin golipid-were isolated from the visceral samples of giant ezo scallop Patinopecten yessoensis by successive column chromatographies (anion-exchange and silicic acid columns). The contents of the isolated lipids were analyzed by gas chromatography and enzymatic methods. The carbohydrate moieties of the cerebroside were galactose (82%) and glucose (18%). The main fatty acids were palmitic, oleic, and stearic acids. Thin layer chromatography analysis of purified phosphosphingolipid fraction after treatment with Clostridium perfringens phospholipase C revealed 2-aminoethyl phosphonate and ceramide, thereby indicating that the chemical structure of the phosphosphingolipid was ceramide 2-aminoethylphosphonate. The main fatty acids in this phospholipid were palmitic acid, branched margaric acid, and 2-hydroxy palmitic acid.
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KJ00006066599
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